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steponeplus sequence detection system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher steponeplus sequence detection system
    Steponeplus Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/steponeplus+sequence+detection+system/steponeplustm+real+time+pcr+system/pmc12056539-70-27-31
    Average 90 stars, based on 1 article reviews
    steponeplus sequence detection system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Biallelic variants in the COQ4 gene caused hereditary spastic paraplegia predominant phenotype
    Article Snippet: Synthesis of total cDNA was carried out using a PrimeScript RT reagent kit (Takara) according to the manufacturer's protocol. .. We performed Quantitative real‐time PCR (qPCR) experiments using a SYBR Premix Ex Taq Kit (Takara) and an ABI StepOnePlus sequence detection system (Thermo Fisher Scientific). ..

    Article Title: Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection.
    Article Snippet: Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty.. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty.. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive.

    Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway
    Article Snippet: .. Thermocycling conditions were as follows: Initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. A StepOnePlus sequence detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.) was used to perform the qPCR tests in triplicate. ..

    Article Title: Streptokinase reduces Streptococcus dysgalactiae subsp. equisimilis biofilm formation
    Article Snippet: .. The real-time PCR amplification was performed with iTaq Universal SYBR Green Supermix kit (Biorad) using a StepOnePlus sequence detection system (Applied Biosystems). ..

    Sequencing:

    Article Title: Biallelic variants in the COQ4 gene caused hereditary spastic paraplegia predominant phenotype
    Article Snippet: Synthesis of total cDNA was carried out using a PrimeScript RT reagent kit (Takara) according to the manufacturer's protocol. .. We performed Quantitative real‐time PCR (qPCR) experiments using a SYBR Premix Ex Taq Kit (Takara) and an ABI StepOnePlus sequence detection system (Thermo Fisher Scientific). ..

    Article Title: Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection.
    Article Snippet: Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty.. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty.. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive.

    Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway
    Article Snippet: .. Thermocycling conditions were as follows: Initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. A StepOnePlus sequence detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.) was used to perform the qPCR tests in triplicate. ..

    Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway.
    Article Snippet: chondrosarcoma is the second most common form of primary bone cancer originating from cartilage. chondrosarcoma cells have a high propensity to spread to other organs during the advanced stage, with the lung being a preferred site. although surgery is the most effec‐ tive treatment for chondrosarcoma, it has low efficacy in the metastasis stage.. Antrodia cinnamomea is the source of the triterpenoid antcin K, which exhibits immunomodulatory and anti‐inflammatory properties.. However, the therapeutic function of antcin K on chondrosarcoma has not yet been elucidated.

    Article Title: Ugonin inhibits chondrosarcoma metastasis through suppressing cathepsin V via promoting miR-4799-5p expression
    Article Snippet: .. The mRNA levels and miRNA expression were assessed using the StepOnePlus sequence detection system (Applied Biosystems) . ..

    Article Title: Streptokinase reduces Streptococcus dysgalactiae subsp. equisimilis biofilm formation
    Article Snippet: .. The real-time PCR amplification was performed with iTaq Universal SYBR Green Supermix kit (Biorad) using a StepOnePlus sequence detection system (Applied Biosystems). ..

    Article Title: Metabolomics and serum pharmacochemistry combined with network pharmacology uncover the potential effective ingredients and mechanisms of Yin-Chen-Si-Ni Decoction treating ANIT-induced cholestatic liver injury.
    Article Snippet: Ethnopharmacological relevance: Yin-Chen-Si-Ni Decoction is a classical traditional Chinese medicine (TCM) prescription that is used clinically for treating cholestatic liver injury (CLI) and other hepatic diseases.. However, the material basis and underlying mechanisms of YCSND are not clear.. Aim of the study: To investigate effective components and mechanisms of YCSND in the treatment of CLI using serum pharmacochemistry, metabolomics, and network pharmacology.

    Article Title: Differential Effects of Endurance Exercise on Musculoskeletal and Hematopoietic Modulation in Old Mice
    Article Snippet: RNA samples (1 μg per reaction) from each group were used for preparation of cDNA for RT-PCR using the AmpiGeneTM cDNA Synthesis Kit (Enzo Life Sciences, Farmingdale, NY, USA). .. Power SYBERR Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) was used to detect the accumulation of PCR products during cycling with the ABI StepOnePLUS sequence detection system (Applied Biosystems, Foster City, CA, USA) with the following program: pre-denaturation at 95C for 2 min followed by 40 cycles of denaturation at 95C for 5 s, annealing at 60C for 30 s, and extension at 65C for 30 s. Primers specific to p16 (forward-gtcgcaggttcttggtcact and reverse-tctgcaccgtagttgagcag) and p21 (forwardtgtccgtcagaacccatc and reverse-aaagtcgaagttccatcgcc) were designed using Primer Express 3.0 (Applied Biosystems). ..

    Amplification:

    Article Title: Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection.
    Article Snippet: Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty.. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty.. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive.

    Article Title: Streptokinase reduces Streptococcus dysgalactiae subsp. equisimilis biofilm formation
    Article Snippet: .. The real-time PCR amplification was performed with iTaq Universal SYBR Green Supermix kit (Biorad) using a StepOnePlus sequence detection system (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection.
    Article Snippet: Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty.. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty.. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive.

    Article Title: Streptokinase reduces Streptococcus dysgalactiae subsp. equisimilis biofilm formation
    Article Snippet: .. The real-time PCR amplification was performed with iTaq Universal SYBR Green Supermix kit (Biorad) using a StepOnePlus sequence detection system (Applied Biosystems). ..

    Expressing:

    Article Title: Ugonin inhibits chondrosarcoma metastasis through suppressing cathepsin V via promoting miR-4799-5p expression
    Article Snippet: .. The mRNA levels and miRNA expression were assessed using the StepOnePlus sequence detection system (Applied Biosystems) . ..

    Concentration Assay:

    Article Title: Metabolomics and serum pharmacochemistry combined with network pharmacology uncover the potential effective ingredients and mechanisms of Yin-Chen-Si-Ni Decoction treating ANIT-induced cholestatic liver injury.
    Article Snippet: Ethnopharmacological relevance: Yin-Chen-Si-Ni Decoction is a classical traditional Chinese medicine (TCM) prescription that is used clinically for treating cholestatic liver injury (CLI) and other hepatic diseases.. However, the material basis and underlying mechanisms of YCSND are not clear.. Aim of the study: To investigate effective components and mechanisms of YCSND in the treatment of CLI using serum pharmacochemistry, metabolomics, and network pharmacology.

    Polymerase Chain Reaction:

    Article Title: Differential Effects of Endurance Exercise on Musculoskeletal and Hematopoietic Modulation in Old Mice
    Article Snippet: RNA samples (1 μg per reaction) from each group were used for preparation of cDNA for RT-PCR using the AmpiGeneTM cDNA Synthesis Kit (Enzo Life Sciences, Farmingdale, NY, USA). .. Power SYBERR Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) was used to detect the accumulation of PCR products during cycling with the ABI StepOnePLUS sequence detection system (Applied Biosystems, Foster City, CA, USA) with the following program: pre-denaturation at 95C for 2 min followed by 40 cycles of denaturation at 95C for 5 s, annealing at 60C for 30 s, and extension at 65C for 30 s. Primers specific to p16 (forward-gtcgcaggttcttggtcact and reverse-tctgcaccgtagttgagcag) and p21 (forwardtgtccgtcagaacccatc and reverse-aaagtcgaagttccatcgcc) were designed using Primer Express 3.0 (Applied Biosystems). ..



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